Publications with MonoTrap
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- Odor Aversion and Pathogen-Removal Efficiency in Grooming Behavior of the Termite Coptotermes formosanus
Aya Yanagawa, Nao Fujiwara-Tsujii, Toshiharu Akino, Tsuyoshi Yoshimura, Takashi Yanagawa and Susumu Shimizu. PLoS One. 2012; 7(10): e47412. doi: 10.1371/journal.pone.0047412<< Read Abstract >>Abstract:
The results of biocontrol with entomopathogens in termites have been discouraging because of the strong social hygiene behavior for removing pathogens from termite colonies. However, the mechanism of pathogen detection is still unclear. For the successful application of biopesticides to termites in nature, it would be beneficial to identify substances that could disrupt the termite's ability to perceive pathogens. We hypothesized that termites can perceive pathogens and this ability plays an important role in effective hygiene behavior. In this study, pathogen-detection in the subterranean termite Coptotermes formosanus was investigated. We performed quantitative assays on conidia removal by grooming behavior using epifluoresence microscopy and Y-maze tests to examine the perception of fungal odor by termites. Three species each of high- and low-virulence entomopathogenic fungi were used in each test. The results demonstrated that termites removed conidia more effectively from a nestmate's cuticle if its odor elicited stronger aversion. Highly virulent pathogens showed higher attachment rates to termite surfaces and their odors were more strongly avoided than those of low-virulence isolates in the same species. Moreover, termites appeared to groom each other more persistently when they had more conidia on their bodies. In brief, insect perception of pathogen-related odor seems to play a role in the mechanism of their hygiene behavior.
- Determination of breath gas composition of lung cancer patients using gas chromatography/mass spectrometry with monolithic material sorptive extraction
Wen Ma, Peng Gao, Jun Fan, Yuki Hashi, and Zilin Chen. Biomedical Chromatography, Volume 29, Issue 6, pages 961-965, June 2015<< Read Abstract >>Keywords:
volatile organic compounds; benzene series; gas chromatography/mass spectrometry; monolithic material sorptive extraction
A gas chromatographic-mass spectrometric method with monolithic material sorptive extraction (MMSE) pretreatment was developed to determine the breath gas composition in lung cancer patients. MonoTrap silica monolithic and hybrid adsorbent was selected as the extraction medium during MMSE, given its strong capacity to extract volatile organic compounds (VOC) from exhaled gas. Under the appropriate conditions, high extraction efficiency was achieved. Using the selected ion-monitoring mode, the limit of detection (signal-to-noise ratio 3) for the benzene series was 0.012-2.172 ng L¯1. The limit of quantitation ( signal-to-noise ratio, 10) was 0.042-7.24 ng L¯1. The linearity range of the method was 4-400 ng L¯1. Average recovery of the benzene series at lower concentrations was 65-74% (20 ngL¯1). The relative standard deviation of benzene series contents determined within the linear range of detection was <10% of the mean level determined. Our proposed method is simple, rapid and sensitive, and can be competently applied to determine the breath gas composition of lung cancer patients.
- A study of the suppression of body odour in elderly subjects by anti-fungal agents
C. Ozeki and O. Moro. International Journal of Cosmetic Science, Volume 38, Issue 3, pages 312-318, June 2016<< Read Abstract >>Keywords:
anti-fungal agents; body odour; claim substantiation in vivo/in vitro; formulation hair + body care; squalene oxidation
The suppression of body odour following the use of shampoos or soaps containing the anti-fungal agent miconazole nitrate (MCZ) has been recognized anecdotally. To determine whether MCZ could play a role in the suppression of body odour through inhibiting squalene oxidation.
A prospective study recruited 54 elderly subjects residing in a nursing facility who needed bathing assistance. Subjects bathed with three types of body soap over a 6-week study period (regular soap, sample soap (soap containing MCZ), control soap; 2 weeks per type of soap). Body odour was evaluated based on olfactory assessment of the subjects and their clothing. The subjects and the examiners were blinded to the type of soap (sample or control) being used during the study. An analysis using GC/MS was also carried out to identify the volatile compounds associated with body odour.
- Determination of Chiral Jasmonates in Flowers by GC/MS after Monolithic Material Sorptive Extraction
Wen Ma, Shuai Fu, Yuki Hashi, and Zilin Chen. J. Agric. Food Chem., 2013, 61 (26), pp 6288-6292 | DOI: 10.1021/jf401104g<< Read Abstract >>Keywords:
chiral separation; endogenous jasmonates; gas chromatography/mass spectrometry; jasmonic acids; monolithic material sorptive extraction
A GC/MS method with monolithic material sorptive extraction (MMSE) pretreatment was developed to determine contents of the enantiomers of jasmonic acid and methyl jasmonate in flowers. To optimize MMSE extraction, several MMSE parameters were investigated, including extraction temperature, extraction time, and extraction solvent. Under the optimal conditions, extraction efficiency was good. Using the selected-ion monitoring mode, the limit of detection (LOD, S/N = 3) for methyl jasmonates was 0.257 ng/mL. The limit of quantitation (LOQ, S/N = 10) was 0.856 ng/mL. The linearity range was 1-100 ng/mL. The average recovery of methyl jasmonate at lower concentration was 116.8% (2 ng/mL). The relative standard deviation of methyl jasmonate contents determined within the linear range of detection was less than or equal to 15% of the mean determined level. The proposed method is rapid, sensitive, and competently applied to the determination of jasmonic acid and methyl jasmonate enantiomers in flowers.
- Characterization of key odor compounds in steamed Chinese mitten crab (Eriocheir sinensis) farmed in Chongming
region by monolithic material sorptive extraction-gas chromatography-olfatometry and odor activity value methods.
Gu SaiQi; Wu Na; Zhang JingJing; Ji SiRu; Tao NingPing; Wang XiChang. Journal of Food Safety and Quality 2014 Vol.5 No.3 pp.877-888 ref.23<< Read Abstract >>Abstract:
Objective: To analyze the volatile compounds in different edible parts of steamed male E. sinensis farmed in Chongming region, select the key odor compounds (KOCs) from all the volatiles, and finally offer some theoretical basis to explain its outstanding flavor. Methods: Seven adsorption rods made of Mono-Trap RCC18 were applied to extract the volatile compounds of 5 g crab sample under 100°C for 50 min under a headspace extraction mode. Those volatiles of crab samples were then separated and identified by GC-MS and quantified by an internal standard method. In order to select the KOCs from all the volatiles, a combination of GC-O and OAV methods were also employed. Results: A total of 71 volatiles were finally identified in male E. sinensis farmed in Chongming region, among which 50, 48, 52 and 60 volatiles were identified in the abdomen, claw, leg meat and the gonad part, respectively. 19 KOCs were successfully selected from all 71 volatiles. Among them, 6 main key odor compounds (MKOCs). With an odor intensity value (OIV) over three and an odor activity value (OAV) over ten, were eventually obtained. Trimethylamine (fishy) was the only MKOC in all four parts. Besides, benzaldehyde (almond-like) and (Z)-4-heptenal (fishy) were the MKOC in the abdomen meat and leg meat, respectively. 3 MKOCs were found in the gonad part, including hexanal (grassy), 1-octen-3-ol (mushroom) and nonanal (grassy).
Conclusion: Six MKOCs were verified to have relative high odor activities by both OAV and GC-O methods. Those MKOCs might represent the key odor characteristics of different edible parts of male E. sinensis farmed in Chongming region. However, the task of revealing formation mechanism for these MKOCs remains to be carried out in the future.
- Determination of the effects of different washing processes on aroma characteristics in silver carp mince by MMSE-GC-MS, e-nose and sensory evaluation
Xuxia Zhou, Yunqing Chong, Yuting Ding, Saiqi Gu, , Lin Liu. Food Chemistry, Volume 207:205-13. doi: 10.1016/j.foodchem.2016.03.026<< Read Abstract >>Keywords:
Silver carp; Washing; Monolithic material sorptive extraction (MMSE); Odor activity value (OAV); Gas chromatography-mass spectrometry (GC-MS); E-nose; Sensory evaluation
The present study investigated the volatile compounds of silver carp mince and the effects of washing processes on the integral aroma characteristics and aroma-active compounds (AACs) of the mince. 57 volatile compounds were identified by monolithic material sorptive extraction (MMSE) and GC-MS analysis, and 13 volatile compounds with odor activity values greater than 1 (OAVs > 1) were further selected as AACs contributing primarily to the integral aroma profile of silver carp. Washing methods affected the overall aroma profiles of fish samples by washing away or facilitating the release of AACs but to different extents. Compared with water washing, washing with saline and weak alkaline solution removed more AACs. Washing with water three times (T2) exhibited a relatively stronger removal effect on most AACs compared to washing with water twice (T1). Washing with a high concentration of saline (T4) produced a strong removal effect on AACs. The results of sensory evaluation and electronic nose (e-nose) detection for distinguishing the aroma characteristics of different samples demonstrated good agreement with those obtained by OAV analysis.
- Characterization of important odorants in four steamed Coilia ectenes from China by gas chromatography-mass spectrometry-olfactometry
Lin min Zhao,Wei Wu, Ning ping Tao, Yu qi, Na Wu, Xiao Qin. Fisheries Science 81(5) - July 2015, DOI: 10.1007/s12562-015-0907-2<< Read Abstract >>Abstract:
Odorants were extracted from four Coilia ectenes (Yangtze River-Coilia, East China sea-Coilia, Chao Lake-Coilia and Yellow River-Coilia) by MonoTrap and analyzed by the headspace-monolithic material sorptive extraction technique combined with gas chromatography-mass spectrometry-olfactometry (GC-MS-O). A total of 63 volatile compounds were identified. The results of GC-MS-O analysis associated with odor intensity revealed that trimethylamine (fishy, ammonia-like), 1-penten-3-ol (mushroom, cabbage), N,N-diethyl-formamide (roasted meaty), hexanal (grassy, earthy), ethylbenzene (nutty, floral), (Z)-4-heptenal (fishy, boiled potato), benzaldehyde (almond, metallic), 1-octen-3-ol (mushroom, cabbage), nonanal (oily), and decanal (green, oily) were the important odorants in the four species of Coilia ectenes. Furthermore, trimethylamine and 1-penten-3-ol were common to four Coilia ectenes. TMA (fishy, ammonia-like), 1-penten-3-ol (mushroom, cabbage), N,N-diethyl-formamide (roasted meaty), hexanal (grassy, earthy), (Z)-4-heptenal (fishy, boiled potato), and benzaldehyde (almond, metallic) were included in Yangtze River-Coilia; TMA, 1-penten-3-ol, ethylbenzene (nutty, floral), (Z)-4-heptenal, 1-octen-3-ol (mushroom, earthy), and nonanal (oily) in East China sea-Coilia; TMA, 1-penten-3-ol, ethylbenzene, 1-octen-3-ol and nonanal in Chao Lake-Coilia; and TMA, 1-penten-3-ol, hexanal, (Z)-4-heptenal, 1-octen-3-ol and decanal (green, oily) in Yellow River-Coilia.
- Optimization of Disk Sorptive Extraction Based on Monolithic Material for the Determination of Aroma Compounds from Lantana camara L. by
Gas Chromatography-Mass Spectrometry
Hye-Jin Jang, Hyun-Hwa Son, and Dong-Sun Lee. Bull. Korean Chem. Soc. 2011, Vol. 32, No. 12 4275<< Read Abstract >>Key Words : Lantana camara, Monoliths, Monolithic material sorptive extraction, Aroma, GC-MS
resent study describes the optimization of disk type sorptive extraction using monolithic material (Mono Trap) for the analysis of volatile aroma compounds from Lantana camara L. in combination with gas chromato- graphy/mass spectrometry (GC/MS). Monolithic material sorptive extraction (MMSE) is a new sampling technique using a monolithic hybrid adsorptive disk (O.D. 10 mm, 1 mm thickness) made of high purity silica and activated carbon having a large surface area chemically bonded with octadecyl silane (ODS). The experimental parameters that may influence the MMSE efficiency have been optimized. Linearity, accuracy, precision and detection limits were evaluated to assess the performance of the proposed method. The method was validated with real plant samples of Lantana camara L. Twenty eight compounds including the main representative compounds of a-curcumene and ß-caryophyllene were found in analyzed samples. Results proved that proposed method could be used as a good alternative for the analysis for such volatile aroma compounds in plant samples.
- Comparison of Volatile Flavor Components in Hepatopancreas and Gonads of Raw and Cooked Chinese Mitten Crab
GAO Xian-chu, GU Sai-qi, TAO Ning-ping, WANG Xi-chang, ZHUANG Jing, WU Na, LI Tai-hang. 2014, Vol.35, No.18 , doi:10.7506/spkx1002-6630-201418026<< Read Abstract >>Key Words: Eriocheir sinensis; monotrap; statistical significance analysis; relative odoractive value (ROAV)
The purpose of this work was to compare the characteristic volatile compounds in two edible parts (hepatopancreas and gonads) of raw and cooked male Chinese mitten crab (Eriocheir sinensis) from Yangcheng Lake. The volatile compounds were extracted with a novel adsorbant (MonoTrap) by solid-phase extraction and analyzed by gas chromatography-mass spectrometry (GC-MS). Totally 90 volatile compounds were identified by GC-MS and compared for significant differences. Except aromatic compounds, all seven other chemical groups of the volatile compounds presented significant differences between the hepatopancreas and gonads, as well as between raw and cooked samples of either one. A large number of aldehydes, furans and N-/S-containing compounds were produced during cooking. Among the 90 volatile compounds, 15 compounds were selected as the major volatile compounds with ROAV values of greater than 0.1, and 9 compounds were selected as the prominent aroma-active compounds with ROAV values of greater than 1. In addition, decanal, trimethylamine, nonanal and octanal made a significant contribution to the overall flavor of raw/cooked hepatopancreas and gonads of Chinese mitten crab