space1


QuEChERS Kits



quechers kits from GL Sciences

EN 15662 Method
AOAC 2007.01 Method

QuEChERS Kits


QuEChERS is an acronym for Quick, Easy, Cheap, Effective, Rugged and Safe which exactly explains its purpose. The kit makes the process of detecting pesticides in food samples easier and faster. It has become a common knowledge that pesticides influence the health of people. Different regulations all over the world make it important for the food industry to detect the different pesticides used on their import products. Therefore the QuEChERS kit was developed in 2003 and have become more important ever since.


QuEChERS Bufferd Extraction Kits

EN 15562 Method

Buffered Extraction Kit
4 g MgSO4, 1 g NaCl, 1 g NaCitrate
0.5 g diSodium Citrate Sesquihydrate

P/No: 8010-1001
AOAC 2007.01 Method

Buffered Extraction Kit
6 g MgSO4, 1.5 g NaCitrate

P/No: 8010-1002


QuEChERS dSPE Extract Cleanup kits

  Matter removed
from sample
Examples    EN 15562 Method    AOAC 2007.01 Method
QuEChERS general purpose
General purpose
25 mg PSA
150 mg MgSO4
2 ml - 100/box
P/No: 8010-1003
50 mg PSA
150 mg MgSO4
2 ml - 100/box
P/No: 8010-1005
  - Polar Organic Acids
  - Some Sugars
  - Some Lipids
 ● Apple
 ● Peach
 ● Brocolli
 ● Fresh Peas
150 mg PSA
900 mg MgSO4
15 ml - 50/box
P/No: 8010-1004
400 mg PSA
1200 mg MgSO4
15 ml - 50/box
P/No: 8010-1006
QuEChERS Fruits and vegetables with fat and waxes
Fruits and vegetables with fat and waxes
25 mg PSA, 25 mg C18
150 mg MgSO4
2 ml - 100/box
P/No: 8010-1007
50 mg PSA, 50 mg C18
150 mg MgSO4
2 ml - 100/box
P/No: 8010-1009
  - Polar Organic Acids
  - Some Sugars
  - Lipids
  - Sterols
 ● Oranges
 ● Lemons
 ● Animal Sourced
 ● Cerials
150 mg PSA, 150 mg C18
900 mg MgSO4
15 ml - 50/box
P/No: 8010-1008
400 mg PSA, 400 mg C18
1200 mg MgSO4
15 ml - 50/box
P/No: 8010-1010
QuEChERS Pigmented fruits and vegetables
Pigmented fruits and vegetables¹
25 mg PSA, 2.5 mg GCB
150 mg MgSO4
2 ml - 100/box
P/No: 8010-1011
50 mg PSA, 50 mg GCB
150 mg MgSO4
2 ml - 100/box
P/No: 8010-1013
  - Polar Organic Acids
  - Some Sugars
  - Some Lipids
  - Carotinoides and Chlorophyll
 ● Zucchini
 ● Strawberry
 ● Dried Peas
 ● Tomatoes
150 mg PSA, 15 mg GCB
900 mg MgSO4
15 ml - 50/box
P/No: 8010-1012
400 mg PSA, 400 mg GCB
1200 mg MgSO4
15 ml - 50/box
P/No: 8010-1014
QuEChERS Highly Pigmented fruits and vegetables
Highly pigmented fruits and vegetables¹
25 mg PSA, 7.5 mg GCB
150 mg MgSO4
2 ml - 100/box
P/No: 8010-1015
 
  - Polar Organic Acids
  - Some Sugars
  - Some Lipids
  - Carotinoides and Chlorophyll
 ● Zucchini
 ● Strawberry
 ● Dried Peas
 ● Tomatoes
150 mg PSA, 45 mg GCB
900 mg MgSO4
15 ml - 50/box
P/No: 8010-1016
 
QuEChERS Fruits and vegetables with pigments and fats
Fruits and vegetables with pigments and fats¹
  50 mg PSA, 50 mg C18
50 mg GCB, 150 mg MgSO4
2 ml - 100/box
P/No: 8010-1015
  - Polar Organic Acids
  - Some Sugars
  - Some Lipids
  - Carotinoides and Chlorophyll
 ● Dairy Products
 ● Curry Spices
 ● Baby Food
  400 mg PSA, 400 mg C18
400 mg GCB, 1200 mg MgSO4
15 ml - 50/box
P/No: 8010-1018
QuEChERS Other food
Other food methods
  25 mg C18
150 mg MgSO4
2 ml - 100/box
P/No: 8010-1019
  - Fats
  - Lipids
  - Proteins
 ● Olive Oil
 ● Citrus Oil
 ● Liver
  150 mg C18
900 mg MgSO4
15 ml - 50/box
P/No: 8010-1020
QuEChERS All food
All food types
  50 mg PSA, 50 mg C18
7.5 mg GCB, 150 mg MgSO4
2 ml - 100/box
P/No: 8010-1021
  - All matrix interfering materials
 
  400 mg PSA, 400 mg C18
45 mg GCB, 1200 mg MgSO4
15 ml - 50/box
P/No: 8010-1022
¹ Not for use with planar pesticides

QuEChERS Related Methods:
EN 15662 Method Foods of plant origin - Determination of pesticide residues using GC-MS and/or LC-MS/MS following acetonitrile extraction/partitioning and clean-up by dispersive SPE - QuEChERS-method.
AOAC Method 2007.01 Pesticide Residues in Foods by Acetonitrile Extraction and Partitioning with Magnesium Sulfate.

How do they work?
The functionality of the QuEChERS kit is to extract any interfering matrices from the sample. The first step (explained on the next page) transfers the pesticides from the water to the added acetonitrile phase by applying MgSO4. Lipids, sugars and pigments are the most important interfering matrices. The second step gets rid of these. Again MgSO4 is added to separate any water. PrimarySecondaryAmine removes fatty acids and sugars, GraphiteCarbonBlack removes pigments and sterols. C18 makes sure that the pesticides do not dissolve in the lipid layer that arises in fat fruits and vegetables (sample > 1% fat). The extraction can be done after homogenizing 10 grams of the sample. When working with dried fruits, add some water before homogenizing. Apply the sample to the provided 50 mL test tubes for the first step. Starting the second step can be done by applying the aliquot supernatant to the provided 2 mL or 15 mL centrifuge tubes.

What QuEChERS kit to use?
On the next page one can find the Buffered Extraction Kits. Pay attention to choosing the correct one for the international market you're producing for (either EU or USA). On the back of this folder a list of QuEChERS kits can be found with a description on what fruits and vegetables they should be used with. Again observe the EU and USA method being displayed. Using the wrong QuEChERS kit can lead to a contaminated chromatogram as well as a contaminated column. Make sure you do know what matrices inside the fruits you are dealing with.

More information about QuEChERS.